GeneTex, Inc.

GeneTexModel GTX109639 - Beta Actin Antibody

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beta-Actin antibody detects beta-actin, a cytoskeletal protein with a predicted molecular weight of 42 kDa. Because of its widespread expression, beta-actin is commonly used as a loading control for various protein assays, particularly western blots, to estimate sample protein amount and quality.

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  • Host  Rabbit
  • Clonality Polyclonal
  • Isotype IgG
  • Application WB, ICC/IF, IHC-P, IP
  • Reactivity Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Rice, Xenopus, Treeshrew, Plant, Worm

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A341 , Jurkat , Raji , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , Rat2 , Rat brain , drosophila , Yeast lysate , Candida albicans (sc5314) whole cell lysate , *Rice leaf , *mouse aorta , *H9C2 , *OKT3 , *mouse ileum tissue , *mouse heart tissue , *mouse skeletal muscle tissue , *mouse brain tissue , *human periodontal ligament

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377395)

Predict Reactivity

Rabbit, Bovine, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

Optimal dilutions/concentrations should be determined by the researcher.

  • Application Dilution
  • WB 1:500-1:20000
  • ICC/IF 1:100-1:1000
  • IHC-P 1:100-1:1000
  • IP Assay dependent
  • Not tested in other applications.