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BovineModel MBS825701 - bta-mir-15a Real-Time RT-PCR PCR Kit

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bta-mir-15a Real-Time RT-PCR Detection and U6 Calibration Kit. Host/Reactivities: Reactivity: Bovine. Samples: miRNA, Total RNA. Assay Type: 2-Step qRT-PCR. Detection Range: Seven orders of magnitude. Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA. Applications: Real-time RT-PCR.

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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.

Species Reactivity
Bovine

Samples
miRNA, Total RNA

PCR Assay Type
2-Step qRT-PCR

Detection Range
Seven orders of magnitude

Sensitivity
10 copies of a standard mimics and as little as 25 pg of total RNA

Platform
ABI 7500 System/ViiA 7 System/StepOne/StepOnePlus/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480

Components

  • 50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.
  • 100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.
  • 200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.

Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for 6 months.

Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Other Notes
Small volumes of bta-mir-15a Real-Time RT-PCR pcr kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Related Product Information for
bta-mir-15a Real-Time RT-PCR pcr kit
Detect mature miRNA bta-mir-15a expression normalized by U6 snRNA.
Applications Tested/Suitable for bta-mir-15a Real-Time RT-PCR pcr kit
Real-time RT-PCR
The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
Synthesized standard amplifiction curve (10nM/100pM)

Amplifiction curve in total RNA from 293T/A549 cells

Dissociation curve (Note: the minor peak on the left is the negative control)

Electrophoresis Image (20bp marker)

Precautions
All of MyBioSource`s Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
  • 50 Reactions/$430 +1 FREE 8GB USB
  • 100 Reactions/$615 +1 FREE 8GB USB
  • 200 Reactions/$880 +1 FREE 8GB USB
  • 5x200 Reactions/$4,020 +5 FREE 8GB USB