Txinno Bioscience - Model Ba/F3 - Cell Line Library Platform Technology

In vitro kinase assay using purified enzyme played a fundamental role in developing variety of different kinase inhibitors for many years. Although testing inhibitor compound in this assay is usually robust depending on the assay conditions, it has obvious limitations that are difficult to improve; 1) activity of inhibitor is likely over-estimated because ATP concentration used in assay is lower than the one inside the cells (millimolar range), 2) it is not trivial to purify recombinant protein to have properties, like post-translational modification and so on, that are close to the enzyme produced inside the cells.