3Helix - Model Auto-CHP - Collagen Hybridizing Peptide, Biotin Conjugate for Automated Staining

• CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3]CHP robustly visualizes the total collagen content of tissue sections that have undergone heat-induced epitope retrieval (HIER). The ratio between damaged collagen and total collagen can be a useful metric for evaluating disease/damage progression over time. Additionally, the signal can be quantified using image analysis software including ImageJ for fluorescent quantification (See Application Note)
• The Auto B-CHP incorporates our newest sequence which allows them to be used with automated staining platforms that are “Open” such as the Bond-RXm (Leica), Discovery Ultra (Roche), and Valent (Biocare Medical) systems. The new sequence will not refold over the course of tissue staining and they do not require a pre-activation heating step. The Auto B-CHP is a powerful histology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging. [3] The compatibility with numerous “Open” automated staining platforms enables easy, consistent, and reproducible staining that can be done simultaneously with other stains.
• Auto B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. The biotin label offers flexible detection options, such as non-fluorescence and HRP-enzyme methods, to avoid background and enhance signal.
• Specificity:CHP binds to the unfolded triple-helical chains of all collagen types(e.g., I, II, III, IV, etc).[3,7]

Specification