Taconic - Model Abb (H2-Ab1)- ABBN12 - Constitutive Knockout Model

The Abb mouse was developed in the laboratory of Michael J. Grusby et al. of the Harvard School of Public Health. The model was created by targeting the H2-Ab1 gene in ES-D3 cells and injecting the targeted ES cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to C57BL/6 for 4 generations (N4). Taconic received stock from Tufts University in December 1991, and the mice were backcrossed eight additional generations (N12) to C57BL/6NTac. The mice were derived by embryo transfer and intercrossed to homozygosity.