Scarab Genomics, LLC
C-Flow - Continuous Fermentation Technology
Our patented C-Flow™ continuous fermentation process is designed to produce large quantities of biopharmaceuticals with small scale inexpensive equipment, saving dramatically on costs. The C-Flow™ process was developed using Clean Genome® E. coli strains expressing recombinant CRM197, a carrier protein used to enhance the immunogenicity of poorly immunogenic carbohydrates in conjugated vaccines.
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- Compatible with continuous manufacturing.
- Small scale equipment – 10-liter fermenters can produce up to kilogram quantities: 1-liter fermenters may suffice for personalized medicine.
- Fast process development.
- Uses fully defined minimal medium.
- Continuous manufacturing is the future gold standard of biopharmaceuticals production (Find out more from the FDA)
- Production facility accommodated in as little as 200 square feet.
- Correspondingly smaller scale purification equipment required.
- Multiple process variables can be tested in series in a single run.
- Limited scale-up required.
- Faster time to market.
- Reproducible production process, purification simplified.
Production levels of CRM197 using Clean Genome® E.coli host versus other traditional production hosts
- C-Flow™ with Clean Genome® E. coli ferments indefinitely, maintaining high levels of protein production
- Traditional strains used in fed-batch fermentation support C-Flow™ protein production for only 1-2 days
Consistent production levels of CRM197 in C-Flow™ over a range of process run times
Reproducible production facilitates predictable results
Continuous fermentations of up to 65 days have been achieved
- Longer runs are more beneficial than a fed-batch approach which uses a single production run in very large vessels (> 1,000 litres) over several days