Model Bilirubin Total - DPD. Colorimetric

Bilirubin (both conjugated and unconjugated) couples with the diazo reagent in the presence of a surfactant to form azobilirubin. The intensity of color formed is proportional to the bilirubin concentration in the sample tested. The increase of absorbance at 546 nm is directly proportional to the total bilirubin concentration.