HUMIMIC ActSense - Electrifying Combination Device of Action and Sensatio

While simple TEER measurements have been successfully employed to assess the junction dynamics and barrier integrity of endothelial or epithelial models, the HUMIMIC ActSense additionally carries out electrical impedance spectroscopy techniques to probe the electrical footprint of a cultured tissue, allowing, e.g., the evaluation of the growth, spreading, and differentiation of different cell types at different maturation stages.

The simultaneous recording of the cells’ electrical activity is implemented by 8 differential (16 single ended) sensing sites, which allows the sensing, e.g., of cardiac action potential, for a better understanding and assessment of heart tissue behavior and health. The multiple stimulation profiles can be used, e.g.,  to initially promote the maturation, and eventually pacing the contractions of mature cardiomyocytes.

Impedance spectroscopy – TEER

• 1x channel / 8x channel through multiplexing.
• Single-frequency (TEER): 96-/24-well.
• Multi-Frequency measurements (impedance spectroscopy).
• Frequency Range: 10 Hz-100 kHz.
• Frequency Resolution: 0.1 Hz.

ECG – EMG – EEG

• Brain (EEG), muscle (EMG), and heart (ECG) activity recording.
• 8x differential channel (16x single ended sensing sites).
• 24-Bit channel resolution: down to micro Volts.
• Gain: 1x, 2x, 4x, 6x, 8x, 12x, 24x.
• Data rates: up to 16 ksps.

Stimulation

• 1x channel / 8x channel through multiplexing.
• Multiple voltage stimulation profiles (square, sine, train of impulses).
• Stimulation frequency: 0 Hz - 10 MHz.
• Cyclic stimulation possible.
• Gain: 1.5x, 2x, 2.5x, 5x.
• Attenuation: 1x, 10x, 100x, 1000x.