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NovaLisa - Model Adenovirus IgG - ELISA Test Kits
The Adenovirus IgG-ELISA is intended for the qualitative determination of IgG class antibodies against Adenovirus in human serum or plasma (citrate, heparin).
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added.
This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
Disease
- Infection of the respiratory system Infection of the eye Infection of the urogenital area Other infections: New born enteritis, meningitis
Symptoms
- Cough, runny nose, mucus production, fever
- Foreign body sensation, itching and irritation, redness and edema, photophobi
- Dysuria / Alguria - pain and burning during watering Pollakiuria - Frequent urination with low urine
- Hematuria- presence of red blood cells in the urine
Mechanism of Infection
- Contact with infected material. Infection mainly by droplets and smear. Often in connection with swimming pools (inadequate chlorination).
- Diagnostic Sensitivity: 100.0 % (95%-width of CI: 95.2% .. 100.0%)
- Diagnostic Specificity: 90.91 % (95%-width of CI: 70.84% .. 98.88%)