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- NeuroSilver - Model NDT103 - FD Kit
NeuroSilver - Model NDT103 - FD Kit
This Kit I is designed for the detection of degenerating neurons in fixed tissue sections of the central nervous system from experimental animals. The principle of this kit is based on the finding that some components of the neurons undergoing degeneration, such as lysosomes, axons, and terminals, become particularly argyrophilic. Under certain conditions, these cellular elements bind to the silver ions with high affinity. Upon reduction, the silver ions from metallic grains are visible under a light or electron microscope. NDT103 Kit has been widely used in animal studies under various experimental conditions1-16. This kit has proven extremely specific and sensitive for the detection of degenerating neuronal somata, axons, and terminals in both the brain and the spinal cord (see photo below). It is particularly useful for the detection of small numbers of degenerating neurons that may not be demonstrable with routine histopathological techniques.
Detection of neurodegeneration with NeuroSilver Kit (NDT103). A: Section (40-micron) through the dentate gyrus of the hippocampus from a rat injected with kainic acid (10 mg/kg, s.c.), showing degenerating neurons and processes (black) in the polymorphic layer (pl) and the molecular layer (ml), respectively. B: Coronal section (30-micron) through the septum of a rat, killed at 10 days following a unilateral transection of the fimbria. Note degenerating axons (indicated by the arrow) in the ipsilateral fornix. C: Horizontal section (40-micron) through the hippocampal dentate gyrus of a rat killed at 5 days after the intra-entorhinal injection with aminooxyacetic acid. Note numerous degenerating axon terminals (arrowheads) in the middle zone of the molecular layer (ml), the terminal field of entorhinal neurons killed by the drug injection (for details, cf. Neuroscience 82:1165, 1998). D: Coronal section (40-micron) through the lateral geniculate nucleus of a rat killed at 5 days after a unilateral visual cortex aspiration. Note dense degenerating axon terminals in the subgeniculate nucleus (SubG) (Section courtesy of Dr. E.-Y. Chen, Rush University [D])
Detection of neurodegeneration in the rat brain of an epilepsy model. 40-micron cryostat section through the cortex of a rat used as an animal model of epilepsy. Note degenerating neurons (black) in the deep layers.
NDT103 Kit has also been proven to be very sensitive and reliable for the detection of amyloid plaques in the brain of transgenic mice (see photos below).
Detection of neurodegeneration and amyloid plaques in the mouse brain of a model for Alzeimer`s disease. 40-micron cryostat section was cut coronally through the dorsal hippocampus of a transgenic mouse used as a model for studing Alzeimer¡¦s disease. The section was processed for detecting both neuronal damage and amyloid plaques with NDT 103 Kit. Note silver-stained plaques in both the hippocampus and the cortex.
High mignification of the hippocampal CA1 area as shown above. Note that in addition to silver-stained amyloid plaques, numerous degenerating fibers are also present in the corpus callosum.
In addition, this kit may be used for demonstrating neurodegeneration and/or amyloid plaques in tissue sections that have been processed for immunohistochemistry (see photos below). The procedure of NDT103-1 Kit takes approximately 1 hour.
Detection of neurodegeneration in the rat brain of a model for Huntington`s disease. 40-micron cryostat section was cut coronally through the striatum of a rat that received a focal injection of quinolinic acid. The section was processed first for bcl2-immunoreactivity and then for detecting neuronal damage with NDT103 Kit. Note that degenerating neurons (black) intermingle with survived bcl2-positive neurons.
- Solution A (500 ml)
- Solution B (500 ml)
- Solution C (500 ml)
- Solution D (500 ml)
- Solution E (2 ml)
- Solution F (3 ml)
- Solution G (5X) (500 ml)
- Glass Specimen Retriever (2)
Materials Required, but Not Included:
- Double distilled water
- Tissue culture plates (6-well)
- Histological supplies and equipment, including gelatin-coated microscope slides, glass coverslips, hair brush, staining jars, xylene or xylene substitutes and a light microscope.