Glomerulus
Podocyte model for assessing glomerular toxicity. Newcells now offers the first fully differentiated, primary podocyte model derived from fresh kidney tissue for the in vitro assessment of drug-induced glomerular toxicity. The effect of a drug on the glomerular filtration barrier and podocyte glomerular permeability can now be easily evaluated in vitro. Podocyte injury and proteinuria can be modelled in vitro at high throughput (96-well format) and assessed by measuring podocyte damage biomarkers, TEER and podocyte permeability. Podocytes maintain the glomerular filtration barrier and similarly to proximal tubule cells, can be damaged by drugs. Drug-induced glomerular toxicity occurs progressively. First podocyte injury leads to cellular dedifferentiation causing perturbation of the podocyte monolayers architecture.
This process in turn results in protein leakage as well as increased levels of protein in the urine, proteinuria, which can cause secondary renal tubular damage and chronic kidney disease (CKD).
- Permeability assay: performed with 70 kDa FITC-dextran
- Cell viability: ATP and TEER measurements
- Biomarker protein quantification
- Inflammation: IκBα degradation, NF-κB translocation
- ER stress and apoptosis: features of diabetic nephropathy
- Fibrosis (Glomerulosclerosis): ECM deposition
- 96 well Transwell® plates for podocyte renal toxicity service
- Podocytes
- Multi-cell type model with endothelial and mesangial cells (coming soon)
- Human
Accelerate your lead compound selection by understanding their mode of action in functional glomerular tissue
- Recapitulate the function of the glomerular filtration barrier
- Evaluate your compounds effect on glomerular toxicity
- Derived from fresh kidney tissue, expressing all podocyte markers