

EndoClear - Model Plus - Human Recombinant a-Synuclein Protein
α-Synuclein is a major component of Lewy bodies in the affected neurons in Parkinson`s disease. This protein has a mass of 14.5 kDa (140 amino acids long) and consists of a conserved degenerative amino-terminal domain and an acidic carboxyl-terminal with higher sequence divergence. α-Synuclein is predominantly expressed in brain, specifically in cerebellum, thalamus, neocortex, hippocampus, and striatum regions. Other tissues express α-Synuclein at very low levels. The physiological role of α-synuclein is not yet well understood. However, the presence of imperfect KTKEGV lipid interacting repeats suggests that it may be involved in synaptic vesicle homeostasis.
*For more stringent applications, EndoClear™Plus offers the lowest endotoxin research grade alpha-synuclein protein available in the market.
Full-length Recombinant human alpha-synuclein (GenBank Accession # NP_000336) was expressed in E. coli., and purified from bacterial lysate using proprietary method. Endotoxin was further removed by proprietary technique. Molecular weight of the recombinant α-synuclein protein is 14.5 kDa.
- Chemistry
- UniProt number: P37840
- Molecular Mass/ Weight: 14.5 kDa
- Modification
- Conjugation: Unconjugated
- Quantity & Purity
- Concentration: none (lyophilized)
- Purification Method: Purified
- Purity: ≥95% by SDS-PAGE
- Endotoxin content: < 0.001EU/ug protein
- Storage & stability
- Form: Lyophilized
- Storage Buffer: The purified a-synuclein is supplied as lyophilized powder nearly salt free.
- Storage Conditions: Store at 2-4 ºC for immediate use within 1 week or at –80 ºC for up to 12 months. Avoid repeated freeze-thaw cycles
- Activity
- Application:
- Aggregation/Fibrillation ELISA
- Western Blotting
- Biomarker Target: Synuclein
- Specificity: human a-synuclein (1-140)
- Research Area: Neuroscience
- Sub-category Research Area: Parkinson`s Disease
- Usage: Research use
- Source
- Host: E. coli
- Source / Species: human
- Codes
- Code Nacres: NA.27