Dextramer - In Situ Staining
Visualize the Landscape of Tissue-Specific Immunity. Tracking T cells in the tissue where they exert their function can advance our understanding of T-cell immunity. MHC I Dextramer® for in-situ staining reagents can detect spatial distribution, localization, and abundance of antigen-specific T cells in different tissue sites in health and disease.
Antigen-specific T cells in tissue sections can be visualized by direct or indirect immunohistochemistry (IHC) methods. Direct visualization uses the conjugated fluorophores on the MHC I Dextramer® reagent, while indirect visualization uses antibody staining directed against the fluorophore on the MHC I Dextramer® for signal amplification. MHC I Dextramer® for in-situ staining reagents provide T-cell staining in fresh samples, paraffin-embedded (FFPE), and frozen sections.
Solid tissue like tumors and lymphoid organs have different structures and spatial distributions of infiltrating T cells, often challenging to identify.
Immudex offers the Dextramer® In-Situ Staining Kit to optimize T-cell staining in solid tissue sections by IHC and comprises three Dextramer® reagents where:
- Each Dextramer® carries the same specificity (e.g., HLA-A*0201/ELAGIGILTV)
- Each Dextramer® has a different number of MHC-peptide complexes, providing reagents with low, medium, or high avidity, respectively
- Each Dextramer® is FITC labeled for direct visualization by fluorescent microscopy
By utilizing the Dextramer® In-Situ Staining Kit, you can identify the reagent optimal for your tissue type and experimental setup. Optimization may also be required for other steps of the IHC procedure, e.g., tissue preparation and antibodies.
Immudex manufactures Dextramer® for in-situ staining reagents made with MHC class I alleles, as listed in the table below. Customized Dextramer® for in-situ staining reagents are easily made with MHC alleles from our catalog and peptides forming stable complexes with the MHC molecule.
