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Expression Systems, LLC
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Expression - Model ESF AF - Insect Cell Culture Medium, Animal Free

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ESF AF™ Insect Cell Culture Medium is an Animal Origin-Free formulation and is a complete serum-free, protein-free medium developed for robust cell growth, protein expression and baculovirus vector production for a wide range of insect cells including Sf9, Sf21, Tni, and Drosophila S2. ESF AF medium is comparable to ESF 921 for growth and expression. ESF AF contains L-Glutamine and Kolliphor® P188 (Pluronic® F-68).

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Expression Systems’ ESF AF is an animal origin-free formulation for insect cell culture. It has been thoroughly tested on Sf9, Sf21, Tni (High FiveTM) and S2 cell lines and is comparable to ESF 921 for growth and expression. ESF AF is the logical choice for further manufacturing under cGMP/FDA regulatory compliance and has a Drug Master File with the US FDA.
  • 100% animal component-free
  • Comparable to ESF 921 medium for robust cell growth and expression
  • Sf9 cell densities of 20 x 106 cells/ml or greater    
  • Ideal for scale-up manufacturing
  • Works with a wide variety of baculovirus and host cell platforms
  • Optimized for applications such as production of sub-unit vaccines, virus-like particles and gene therapy vectors (rAAV — all serotypes)
  • Available in 1L bottles
  • Complete, ready-to-use, contains L-glutamine
  • Adapted cells available in suspension
  • Inquire about custom formulations, packaging for bioreactors and master cell bank availability for licensing
  • Media type: ESF AF Insect Cell Culture Medium, Animal Free
  • Cell lines: Spodoptera frugiperda (Sf9, Sf21), Trichoplusia ni (Tni and High Five™), and Drosophilia melanogaster (S2)
  • Platform compatibility: Functions with BestBac™, flashBAC™, Bacmid, Bac-to-Bac®, BacMam™, MultiBac™ and other BEVS platforms
  • Shipping condition: Ambient
  • Storage condition: 2-8°C, protect from light
  • Use-by date: One year from date of manufacture