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OriGene - Lentivirus shRNA, Plasmids and Ientiviral Particles
OriGene offers pre-made lentiviral shRNA plasmid kits containing 4 sequence-verified expression cassettes that target your gene of interest. Each lentiviral cassette contains three major functional elements: shRNA, a puromycin selection marker & a tGFP reporter. With a guaranteed knockdown of ≥70%, these kits and our ready-to-use lenti-shRNA particles are a great option for studying long-term gene knockdown.
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- Potent shRNA design ( learn more )
- Genome-wide coverage: Human, Mouse & Rat
- Safety: 3rd generation system with improved biosafety
- Trusted vendor for lentiviral products: view citations
pGFP-C-shLenti vector
- Third generation Lentivector
- Chloramphenicol marker for bacterial selection
- Three major functional elements within the 5’LTR and 3’LTR. All of them can be packaged to viral particles and transduced to many cell lines.
- shRNA expression cassette under U6 promoter
- Puromycin resistant for mammalian cell selection
- tGFP gene as reporter.
The shRNA expression cassette
- 29mer hairpin with a 7bp loop.
- U6 driven expression
- A Pol III termination sequence (TTTTTT) immediately downstream of the hairpin.
- The gene-specific shRNA cassette is sequence-verified to ensure its match to the target gene.