AptaFluor -Â Model SAH - Methyltransferase Assay Kit
AptaFluor SAH Methyltransferase Assay directly measures SAH produced by methyltransferase enzymes during methylation. These enzymes methylate a variety of substrates using S-adenosylmethionine (SAM) as the methyl donor, leaving the common product S-adenosylhomocysteine (SAH). These SAH measurements allow researchers to effectively determine the activity of the methyltransferase enzyme. The assay provides a powerful tool to screen compound libraries for methyltransferase modulators to help find new therapies for disease. The kit comes complete with detection reagents required to measure activity. Methyltransferase enzyme is not included in the inhibitor screening assay kit.
The AptaFluor SAH Methyltransferase Activity Assay determines methyltransferase enzyme activity by directly measuring SAH (S-adenosylhomocysteine) produced during the methylation of a variety of substrates. By directly detecting SAH in a homogenous format, it is possible to perform screening and enzymatic studies with virtually any methyltransferase.
AptaFluor Methyltransferase technology uses a natural occurring aptamer, or riboswitch, that selectively binds with SAH. The exquisite affinity and selectivity of the riboswitch enables screening and profiling of methyltransferases with unparalleled sensitivity.
The AptaFluor Assay is available with TR-FRET readout. It is a simple mix-and-read format. Perform your enzyme reaction, add the detection reagents, and measure. The simplicity of the system yields robust results that also makes it extremely amiable to HTS.
Ultra-Sensitive
The most sensitive HTS methyltransferase assay available with an LLD of 0.6 nM SAH. This dramatically reduces enzyme usage and allows the assay to be run at or below Km for SAM.
Easy-to-Use, Mix-and-Read, HTS-Ready Assay
Run your enzyme reaction, add quenching reagent, add AptaFluor reagents, and read your plates. The AptaFluor Assay is compatible with 96, 384, and 1536-well formats.
Robust Assay Yields Quality Data
Excellent Z’ under a variety of assay conditions.
Stable Detection of Methyltransferase Activity
Outstanding reagent and signal stability reduces assay variability and allows for automated workflows.
Interested in moving your program forward, but don`t want to bring an assay in-house? Our scientists can help! BellBrook scientists will use their extensive biochemistry and enzymology expertise to work with you and accelerate your methyltransferase program.
- Inhibitor Screening – To identify or confirm activity with the target.
- Inhibitor Potency Profiling – Dose-response with target and/or related proteins. Fast IC50 results.
- Residence Time Measurements – Determination of koff using ‘jump dilution’ enzymatic assay method.
- Mechanism of Action Studies – Kinetic analysis to define the mode of inhibition.