thrombomiRs - microRNA Biomarkers of Platelet Function

Circulating platelet microRNAs (thrombomiRs®) are novel biomarkers for intrinsic and on-treatment platelet reactivity. thrombomiRs® are secreted from platelets during their activation and can be detected in plasma and serum. Commonly used platelet function tests e.g. LTA, VerifyNow® or PFA-100®, require fresh blood and agonists. Therefore, they clearly lack standardization and their analytical variability may induce misinterpretations. The thrombomiR® kit enables an in vivo measure of platelet function, independently of the activation pathway.

Most popular related searches

platelet function

in vivo measurement

One-stop-shop solution to start microRNA platelet function research
Contains all quality controls required to perform high quality microRNA biomarker research and publication
1 kit allows to 10 platelet microRNAs in up to 48 samples
Cross-species application is possible due the high sequence conservation.
Fast and simple data analysis using the thrombomiR analysis toolkit

Frozen platelet poor plasma (PPP) aliquots of at least 200 µl
qPCR instrument (read the manual to find out which instruments are supported)

High throughput: One kit is sufficient for the analysis of 48 platelet poor plasma (PPP) samples
Low sample volume: 200 µL PPP are recommended (lower volumes can be used but require pre-testing), (-70°C or lower) PPP samples can be used (stability for several years has been demonstrated)
Reduced hands-on time: primer coated 96 or 384 well plates
10 thrombomiR^®s and 6 controls/sample are measured per sample
Fast and simple data analysis: the web-based thrombomiR^® software application enables easy data normalization and quality control

Software application

The thrombomiR^® analysis toolkit enables

standardized analysis of qPCR data
import: upload of raw data files from qPCR instrument and automatic Cq-value calling
quality control: inspect amplification curves, melting temperature, spike-in and hemolysis controls
normalization: correction of technical variance and optional imputation of missing values
export: export normalized Cq-values of all samples and combine automatically with clinical parameters (i.e. age, gender, etc.)

The thrombomiR^® analysis toolkit and further information will be made available to all registered customers of the thrombomiR^® kit.

thrombomiRs - microRNA Biomarkers of Platelet Function

Benefits

Assay Requirements

Product Description