Virasoft - Model PD-L1 - Programmed Cell Death Protein
Programmed cell death protein 1 (PD-1; also called CD279) is one of the co-inhibitory receptors that is expressed on the surface of antigen-stimulated T cells [1]. Normally, PD-L1 expression can be detected on hematopoietic cells including T cells, B cells, macrophages, dendritic cells, mast cells and healthy tissue cells. But PD-L1 can be expressed by tumor cells and tumor stroma. Interaction tumoral PD-L1 with PD-1 stimulates PD-1-mediated T cell inhibition and down regulation of immune response. PD-L1-PD-1 pathway has proven value as a therapeutic target in a large number of malignancies including non-small cell lung cancer [2]. Immunohistochemical staining is a standardized approach to PD-L1 expression in order to distinguish for patients who may benefit from therapies [3]. Virapath PD-L1 analysis provides accurate quantification of positive cell proportion
But PD-L1 can be expressed by tumor cells and tumor stroma. Interaction tumoral PD-L1 with PD-1 stimulates PD-1-mediated T cell inhibition and down regulation of immune response. PD-L1-PD-1 pathway has proven value as a therapeutic target in a large number of malignancies including non-small cell lung cancer [2]. Immunohistochemical staining is a standardized approach to PD-L1 expression in order to distinguish for patients who may benefit from therapies [3]. Virapath PD-L1 analysis provides accurate quantification of positive cell proportion.
PD-L1, PD-1, Immunotherapy, Lung Cancer, NSCLC, Image Analysis
To obtain H and DAB colour channels of the image the color deconvolution algorithm is used. Nuclei are detected on H channel and nuclei border pixels are evaluated one by one. Nuclei border pixels on DAB channel are analysed according to positive limit value and rate positive or negative.
- Positive cell count
- Negative cell count
- Positive cell percentage
- Step 1: View a PD-L1 stained lung cancer whole slide image at ViraPath
- Step 2: Outline tumor either manually or automatically using Virapath Tissue Segmentation algorithm
- Step 3: Select the PD-L1 analysis and calibrate the parameters
- Step 4: Run the analysis