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Protein - Biuret Method
FromBiodiagnostic
In the presence of an alkaline cupric sulfate, the protein produces a violet color, the intensity of which is proportional to their concetration.
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Serum, or plasma, Hemolysis will interfere with the test
Adults : 6.5 – 8.7 g /dL .
Children : 5.5 – 8.5 g / dL .
Neonates : 5.3 – 8.9 g / dL .
Mix well, incubate for 10 min. at 37°C. Read the absorbances of the sample (ASample ) and standard (Astandard ) against reagent blank at 550 nm. ( 520 - 570 nm) . Color stable for one hour. Linearity up to 10 g / dL .
The reagents are stable up to the expiry date specified when stored at +4 to +8 °C away from light.