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Chema DiagnosticaModel Adenosine Deaminase (ADA) FL - Reagent for Quantitative In Vitro Determination

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Reagent for quantitative in vitro determination of adenosine deaminase in biological fluids (serum) and intended as aid to diagnosis of tuberculous pleurisy and ADA severe immunodeficiency disease. The IVD devie can be used both in manual or on automatic analyzers. The product is intended for professional use in clinical laboratories.

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ADA enzyme converts adenosine to inosine. which starts a sequence of enzymatic reactions mediated by PNP (Purine Nucleoside Phosphorylase) and XOD (Xanthine Oxidase), leading to hydrogen peroxide (HsO?). This compound reacts with TOOS (N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methyianiline) in the presence of peroxidase, to form a chinone compound. Absorbance increase per unit time, measured at 546. is proportional to the concentration of ADA in the sample.