Chema Diagnostica - Model Albumin - Reagent for Quantitative In Vitro Determination

Plasma levels of albumin, because they depend on protein intake, are frequently used to assess nutritional status. Moderate to large changes in plasma concentration of albumin have significant effects on the relative amounts of the bound and free concentrations of the ligands it carries, as a consequence on the metabolism of endogenous substances such as calcium, bilirubin, and fatty acids and on the effects of drugs and hormones.

Albumin and BCG are followed to bind at pH 4.2, and absorption of the BCG-albumin complex is determined spectrophotometrically at 628 nm. At pH 4.2, albumin acts as a cation to bind the anionic dye.