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- Recombinant Gra1, Toxoplasma Gondii
Recombinant Gra1, Toxoplasma Gondii
Recombinant Toxoplasma gondii Gra1 is expressed in E. coli as a monomeric, non-glycosylated polypeptide chain consisting of 175 amino acids with a molecular mass of 18600 Da. The product represents the complete sequence of the mature Gra1, but lacks the signal peptide of the native protein.
The integrity of the plasmid is controlled by restriction analysis and DNA sequencing. The expression product is characterized by N-terminal protein sequencing, SDS-PAGE (app. m.w.) and immunodetection of the Western blotted protein. In addition, correct reactivities compared to previous antigen lots with defined human and animal sera (negative, low, medium and high positive) are mandatory.
Storage and Shelf Life
At -80°C 10 years from the date of production
Avoid repeated freeze-thaw cycles
- p24, dense granule protein 1
- Source: Genetically engineered Escherichia coli
- Stabilizers: None
- Preservatives: None
- Diluent: Antigen specific solution, does not contain detergents, denaturants (e.g. urea, GmHCl) or reductants. Antigen lots in PBS are prepared on request only.
- Purification: Ion Exchange Chromatography
- Purity: 98%, analysed by SDS-PAGE (reducing and non-reducing) followed by Coomassie-Blue staining and immunoprobing of Western blotted proteins with anti-E.coli serum (DAKO)
- Endotoxin: Not determined
- Protein concentration: Determined spectrophotometrically at 280nm (ext. coeff. 3840) and densitometrically by Advanced Image Data Analyzer software (Raytest) in relation to BSA standards