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TrypsiNNex - Recombinant Porcine Trypsin for Biopharmaceutical Manufacturing
TrypsiNNex® is an innovative, animal-free recombinant porcine trypsin designed for biopharmaceutical manufacturing. This enzyme is recognized for its consistency and reliability in various production processes including cell dissociation, influenza virus production, and recombinant insulin manufacturing. Engineered to provide high purity and quality, TrypsiNNex® minimizes the risks associated with viral contamination from animal-derived materials. It is expressed in E. coli as an inactive product to prevent early degradation, ensuring a stable β-trypsin content above 70%, critical for maintaining enzymatic activity and specificity. The enzyme's autolytic pathway is carefully managed to sustain its functional form, crucial when processing precursor proteins like pro-insulin. Manufactured under GMP conditions as per IPEC guidelines, TrypsiNNex® offers high enzymatic activity and maintains stability for 36 months at -20ºC. Its formulation includes 10 mM HCl and 20 mM CaCl2, with low microbial contamination levels, making it suitable for stringent biopharma environments.
TrypsiNNex is an animal-free, recombinant porcine trypsin that delivers exceptional performance and consistency for highly regulated biopharma manufacturing processes.
Trypsin is a highly specific protease widely used in biopharmaceutical production, ranging from cell dissociation, virus production, to modification of proteins and virus particles.
TrypsiNNex® offers the following benefits:
Animal-free
Eliminate the risk of viral contamination from raw material
High purity
Achieve high process consistency owing to consistently high β-trypsin content
High quality
Easy qualification or transition from your existing trypsin product, supported by extensive documentation and support from a preferred pharma grade supplier
The animal-free, recombinant TrypsiNNex® sequence is identical to porcine Trypsin and can be used as a direct replacement when converting to animal-free manufacturing. TrypsiNNex® is expressed in E. coli as an inactive product. The trypsin remains inactivated during both the upstream and downstream manufacturing process, minimizing the level of auto degradation before final stabilization and packaging, thereby preserving a high content of β-trypsin.
Trypsin has intrinsic autolytic activity and is degraded in steps, where β-trypsin is converted into α-trypsin through proteolytic cleavage (red scissors).
This process continues until the trypsin is inactivated. Intermediate forms such as Ψ-trypsin display unspecific activity, which is undesirable. Thus, a high β-trypsin content is a quality indicator that ensures consistent activity and high specificity.
TrypsiNNex® has a measured β-trypsin of typically well above the minimum specifications at 70% ensuring a high activity and consistent proteolytic pattern, which is critical when processing precursor proteins, such as pro-insulin.
- Cell culture-based influenza virus production using TrypsiNNex®
- Efficient and consistent cell dissociation using TrypsiNNex®
- Consistent and effective production of Recombinant Insulin using TrypsiNNex®
- Cell culture-based influenza virus production using TrypsiNNex®
- Efficient and consistent cell dissociation using TrypsiNNex®