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  5. TaqMan - SARS-CoV-2 Mutation Panel

TaqMan - SARS-CoV-2 Mutation Panel

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Choose from a menu of verified real-time PCR assays to build your own custom Applied Biosystems TaqMan SARS-CoV-2 Mutation Panel, and get the results you need today, while preparing you for what’s to come. This scalable solution lets you run a few or hundreds of samples to identify one or many mutations—all on your current real-time PCR instrumentation.

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  • Customizable-build your own custom panel from a menu of verified real-time PCR assays that allows you to identify currently relevant SARS-CoV-2 mutations and adapt quickly as additional mutations and variants emerge
  • Convenient-use your current real-time PCR instrumentation to conduct follow-up testing of SARS-CoV-2 samples
  • Scalable-run a few or hundreds of samples to identify for one or many mutations
  • Unique, streamlined workflow-combines our gold-standard TaqMan SNP Genotyping Assays with a 1-step RT-PCR reaction; go from RNA to results in just over 1 hour
  • Targets: Choose from a menu of verified real-time PCR SNP assays to build your custom panel
  • Assay design:
  • Sequence-specific forward and reverse primers to amplify the target sequence region. The reverse primer also primes reverse transcription of the SARS-CoV-2 genomic RNA sequences. Each assay includes two TaqMan minor groove binder (MGB) probes with nonfluorescent quenchers (NFQ):
    • One VIC dye labeled probe to detect the reference sequence
    • One FAM dye labeled probe to detect the mutation sequence
  • Available sizes
    • 375 reactions
    • 1,250 reactions
  • Sample input: RNA extracted from SARS-CoV-2 samples with a CT value of less or equal to 30
  • Turnaround time: 1 hour and 10 min from extracted RNA to results
  • Recommended instruments: Any real-time PCR instrument such as the Applied Biosystems 7500, 7500 Fast Dx, 7500 Fast, QuantStudio 5, and QuantStudio 7 systems
  • Recommended software: QuantStudio Design and Analysis Software v2.5 or later with the Genotyping Analysis Module

Our menu of verified real-time PCR assays provide robust detection of SARS-CoV-2 mutations. Below are results (cluster plots) as viewed using QuantStudio Design and Analysis Software v2.5 with the Genotyping Analysis Module. These cluster plots show very clear discrimination between the wild type samples (red dots along the x-axis) and the mutation samples (blue dots along the y-axis).

Starting with isolated RNA from SARS-CoV-2 positive samples, our unique workflow combines our gold-standard Applied Biosystems TaqMan SNP Genotyping Assays with a one-step RT-PCR reaction to detect whether there are known mutations present in your samples or not.

In this video, we’ll show you how this one-step RT-PCR reaction works and what you can expect your results to look like.

In this short, two-video tutorial, you will learn how to:

  • Set up the instrument software for detecting the SARS CoV-2 mutation panel
  • Analyze the data with the Applied Biosystems Design and Analysis Software v2.5 or later with the Genotyping Analysis Module using the run files from all Applied Biosystems real-time PCR instruments

Tips and tricks for setting up your experiment and analyzing results

In this short video, you will learn:

  1. How to set up Genotyping Analysis in your instrument software
  2. How to set up your method with a reverse transcription step
  3. How to select real-time data in the genotyping analysis settings
  4. How to view results and read cluster plots