Star Dyes
Indirect immunofluorescence staining with abberior STAR dyes coupled to polyclonal anti-mouse secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch shows excellent results in 3-color STED imaging. Staining was performed according to the premix & stain protocol. Vimentin (abberior STAR RED, green), tubulin (abberior STAR 580, magenta) and dsDNA (abberior STAR 460L, blue) were labeled in fixed mammalian cells and visualized with the STEDYCON.
abberior STAR dyes enable background-free labeling in cells or tissue, to get the most out of your sample. They excel with high brightness and photostability, while conjugates of the dyes show an excellent solubility in aqueous buffers as PBS.
abberior STAR L dyes are abberior’s long Stokes-shift dye series. The common characteristic of the dyes is an exceptional long Stokes-shift between the excitation and emission maximum. Our abberior STAR L dyes are designed to provide bright fluorescence for multiplexed imaging. Conjugates of the dyes show a very good solubility in aqueous buffers as PBS to allow labeling of the structures of interest with a good signal-to-noise-ratio.