Model Cat. 49500 - Stool Total RNA Purification Kit
This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen`s Stool Nucleic Acid Collection and Transport Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered.
The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes.
A convenient and rapid method to purify total RNA from small amounts of stool samples
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Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
- Isolate full diversity of RNA from large RNA down to small and microRNAs
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Eliminates PCR inhibitors including humic acids
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High quality RNA for sensitive downstream applications
- Maximum Stool Input: 200 mg (fresh or frozen stool)
- Type of Stool Processed: All types of feces from humans and animals
- Maximum Column Binding Capacity:50 μg
- Maximum Column Loading Volume: 600 μL
- Time to Complete 10 Purifications: 30 minutes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.