LifeSensors - Model UM-0101 - Ubiquitin Affinity Matrix
Based on UBA domains known to possess a high affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs have been demonstrated to protect polyubiquitinated proteins from both deubiquitination and proteasome-mediated degradation, allowing for the detection of relatively low abundant proteins that cannot be detected with current technologies. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply with these terms.
- Tag : GST
- Molecular Weight : 56 kDa
- Physical State : Liquid :
- Quantity : 200 µg and 1 mg
- Concentration : Variable
- Storage : -80°C, avoid freeze/thaw cycles
- Pull down of poly-ubiquitylated proteins from cell and tissue lysates
- Protection of poly-ubiquitylated proteins from both deubiquitylation and degradation by the proteasome
- Up to 1000-fold higher affinity than the single UBA domain
- Avoid overexpression of affinity-tagged ubiquitins for pull downs
- Protect poly-ubiquitin chains without inhibitors specific to the proteasome or deubiquitylase activity
- Replace ubiquitin specific antibodies for enrichment of poly-ubiquitylated proteins