Rabbit TrueBlot - Model 18-8816-31 - Unique Horseradish Peroxidase Conjugated Anti-Rabbit IgG Monoclonal Secondary Antibody

• Description: Rabbit TrueBlot®: Anti-Rabbit IgG HRP - 18-8816-31
• Synonyms: Anti-Rabbit IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
• Tested Applications: ELISA, IP, WB
• Suggested Applications: ChIP, IF  - View References
• Target Species: Rabbit
• Cross Reactivity: Rabbit
• Host Species: Mouse
• Conjugate: Peroxidase (Horseradish) ULTRA
• Clonality: Monoclonal
• Clone ID: eB182
• Format: IgG
• Detection Kit Type: Immunoprecipitation Kit
• Physical State: Liquid (sterile filtered)
• Shipping Condition: Wet Ice
• Expiration: Expiration date is six (6) months from date of opening.
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: Proclin is added as an antimicrobial agent.
• Stabilizer: 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.

Application Note

Rabbit IgG HRP TrueBlot has been tested in ELISA, western blot, and immunoprecipitation and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Rabbit IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mLs/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the immunoprecipitation. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For immunoprecipitation, Anti-Rat IgG beads, or Anti-Rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.

Purity/Specificity

Rabbit TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA.

Storage Condition

Store at -20 °C. This product is guaranteed for 1 year upon receipt, when handled and stored as instructed.