MiraVista Veterinary Diagnostics

Aspergillus Antibody Immunodiffusion

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Clinical Significance: The Aspergillus Antibody Immunodiffusion test is used for the in vitro determination of precipitating antibodies to Aspergillus spp. The test is most useful in assisting with the diagnosis of sino-nasal aspergillosis in dogs.
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Specimens:  Serum, CSF*, Plasma*
*Uncommon specimen includes incomplete validation. A “rare specimen” note will appear on the final report.

Container:
Serum: Collect serum specimens in serum separator tube. Allow blood to clot for 30 minutes, then centrifuge. Send in serum separator tube or transfer tube.
CSF, Plasma: Submit CSF and plasma in a leak-proof plastic tube.

Volume: 0.25mL
Stability: Room temperature = 28 days, Refrigerated = 6 months, Frozen = indefinite

Specimen Rejection

Any specimen types other than serum, CSF, plasma

Methodology

Fungal Immunodiffusion

Limitations

The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.

  • Negative serologic tests may be observed among culturally demonstrable cases, which limits the predictive value of a negative test.
  • Negative serologic results obtained during the first month may warrant repeat testing.
  • Falsely negative results may be obtained when testing immunocompromised patients.
  • Test results cannot be used solely to distinguish between active infection and prior exposure.
  • C-Reactive Protein present in the patient’s serum may interfere with interpretation of results.
  • Cross Reactivity/False Positive: Moderate to Low: Blastomyces, Histoplasma, and Coccidioides

Turnaround Time

Testing performed Tuesday and Friday.

Results Released: Positive: 24 hours; Negative: 72 hours.

Reference Range

Antibody not detected

Interpretative Information

Negative: Antibody not detected

Positive: Antibody detected