ExcellGene - Cell Line Development Services

During the creation of the CHOExpress™ and HEKExpress-293™ systems for CHO and HEK cell line development, we were able to identify highly stable and robust cell lines for scale-up. Therefore, we can now develop cell lines in less than 5 months. Along with USP/DSP development, we excel at adjusting chemically defined media and feeds to deliver 2 to 10 times higher titer than industry standards.

What we do

• Transfect cells very efficiently to integrate the gene of interest DNA into active chromatin
• Generate highly productive recombinant cell pools (> 3 g/L for IgGs)
• Establish clonal CHO cell lines with documented evidence of clonality
• Establish research cell banks and study their stability (>3 months without selection)
• Media, feed and clone screening for identification of optimal match of physiology and genetics for highest yield and quality to obtain 5-14 g/L yields for IgGs and other proteins
• Apply DOE for process optimization
• Execute and develop thorough analytics and specific assay development support
• Develop scalable upstream and downstream processes for transfer in CMOs

It`s in our DNA

ExcellGene has decades of accumulated know-how in protein expression with many mammalian cells including CHO, HEK-293, SP2.0 and NSO. Because of our science-driven approach to cell line development, we provide expert solutions to express any protein of interest, from simple to complex, ensuring high titers.