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MtoZ Biolabs - Model phip-seq-antibody-analysis-service -PhIP-Seq Antibody Analysis Service
Phage Immunoprecipitation Sequencing (PhIP-Seq) is a revolutionary approach that integrates oligonucleotide-encoded peptide pools with phage display libraries and high-throughput DNA sequencing. This technique allows for an in-depth analysis of antibody-peptide binding affinities across a standardized "peptidome," representing the entire protein sequence library. Not only does it measure the binding strength of specific peptides to antibodies, but it also facilitates comparisons across a spectrum of peptides, including control samples without antibodies.
Bourgonje, A. R. et al. Immunity. 2023.
At MtoZ Biolabs, our PhIP-Seq service utilizes in phage display and DNA sequencing technologies to deliver comprehensive insights into antibody diversity and specificity. This service is crucial for discovering disease markers, advancing vaccine development, and enhancing personalized medicine strategies. Our use of oligonucleotide-encoded peptide pools ensures broad coverage of the protein library, improving the detection of antibody binding sites and enhancing the consistency and reproducibility of our analyses.
Our PhIP-Seq platform is pivotal in:
Clinical Research: Identifying new biomarkers by analyzing antibodies in both patients and healthy subjects.|Vaccine Development: Evaluating vaccine efficacy through detailed pre- and post-vaccination antibody analyses.|Antibody Drug Development: Optimizing therapeutic antibodies for specific disease targets.
PhIP-Seq outperforms traditional antibody assays with:
It analyses a broad spectrum of proteins and antigens without relying on conventional protein expression systems.|It offers a significant cost reduction per sample compared to traditional protein microarrays.|The peptides used are of higher quality and longer, ensuring detailed and comprehensive analyses.
For optimal results with our PhIP-Seq service, we require:
1. Types of Samples
We accept various biological fluids, including serum, plasma, urine, and more.
2. Sample Volume
We recommend providing at least 50 microliters per sample to ensure sufficient DNA quantity for high-throughput sequencing
3. Storage and Transport
Samples should be frozen at -80°C and shipped on dry ice to maintain integrity.
4. Sample Quality
Please ensure that the samples are virus-free, sterile, and have not undergone any protein or chemical treatments to avoid affecting the accuracy of the experimental results.
MtoZ Biolabs offers a seamless and precise PhIP-Seq antibody analysis experience, from sample handling to data analysis. Our technology's reliability, precision, and adaptability make us an ideal partner for your antibody analysis needs. Contact us to discover how we can assist in advancing your research or clinical projects with our innovative services.
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com
