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Biomatik Corporation
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Recombinant Protein Production Service (E. coli)

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Biomatik has been proudly serving 10,000+ scientists since 2002. If you cannot find a protein from our extensive protein catalog to suit your research needs, then our custom protein production service team can help. Our custom protein production service utilizes our proprietary codon optimization algorithm and in-house gene synthesis technology to offer customers the best customized research products at affordable prices. Our numbers speak for themselves, with 12 years of extensive experience in custom protein expression and over 95% success rate in E. coli recombinant protein expression projects, you can be sure your protein productions are in good hands.

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E. Coli
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Please check out our custom E. coli protein expression service packages and sample QA report. We offer risk free guarantee for most proteins (10kDa ~ 80kDa) from E. coli. No Protein, No Charge. It is free of charge if we are not able to produce the protein. View select publications featuring custom recombinant protein production by Biomatik:

•  Risk-free Guarantee: No Protein, No Charge! We guarantee to produce the requested protein quantity & purity, or it is free of charge! 
•  Free gene synthesis & subcloning. No setup fee.
•  Biomatik`s tag-free protein production is unlike the traditional tag removal process. Our tag-free protein starts with tag-free protein expression, and purified by SEC and ion exchange to ensure proper native folding. Customers receive high quality protein with no trace of the affinity tag since no tag removal involved in production.
•  If the expressed protein is insoluble (expressed as inclusion body), refolding will be attempted to make it soluble.
•  Protein purity is determined by SDS-PAGE, and quantity is determined by Bradford assay.
•  Protein is delivered in freeze powder whenever possible (in neutral buffer before lyophilization: 1x PBS, pH 7.4). If protein is delivered in solution form, the default buffer is: 1 x PBS, 50% Glycerol, pH 7.4.

Tag-free Protein Production Service from Biomatik
A tag-free protein is very crucial for many R&D experiments. The conventional recombinant protein production introduces different tags (typically His-tag or GST-tag) to carry out protein expression and purification. Unfortunately, these tags cannot all be removed and can create real issues in downstream applications. Unlike traditional post-production tag removal, Biomatik`s tag-free protein production service ensures proper native folding, and no trace of the affinity tag (no tag cleavages). Our customers can now count on us for truly "tag-free" proteins at highly affordable prices.

Endotoxin Removal Service from Biomatik
Endotoxins are associated with the outer cell membrane of gram-negative bacteria such as E. coli. The major component of the outer cell wall membrane is composed of Lipopolysaccharides (LPS) which can elicit a range of inflammatory responses in the mammalian hosts and can often be detrimental to such host systems. It is necessary to remove these endotoxins from the final protein preparation for downstream applications involving in vivo studies or cell-based assays. Biomatik’s Endotoxin Removal Service can be added as part of your custom protein expression service, and we can reduce the endotoxin level to below 1EU/ug with great success.

Why Choose Biomatik?

Biomatik has been trusted among top scientists and researchers from around the world for quality products and custom services since 2002. To date, Biomatik has delivered 70,000+ custom made products to our customers worldwide. Learn why Biomatik has been trusted by thousands of researchers worldwide by viewing our customer testimonials, selected publications and 3,000+ publications citing our fine products and services at Google Scholar.