Lexogen GmbH
- Home
- Companies
- Lexogen GmbH
- Videos
- TECHNOLOGY INTRO: SLAMseq: Thiol-linked ...
TECHNOLOGY INTRO: SLAMseq: Thiol-linked alkylation for the metabolic sequencing of RNA - Video
TECHNOLOGY INTRODUCTION at AGBT 2018: SLAMseq: Thiol-linked alkylation for the metabolic sequencing of RNA
ABSTRACT:
Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, processing and decay. We developed thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAMseq), an orthogonal chemistry-based epitranscriptome-sequencing technology that uncovers 4-thiouridine (s4U)-incorporation in RNA species at single-nucleotide resolution and absolute stoichiometry. In combination with established metabolic RNA labeling protocols and coupled to standard, high-throughput RNA sequencing methods, SLAMseq provides rapid access to RNA expression dynamics. We show that SLAMseq reports the functional RNA polymerase II-dependent transcriptional output, enables the systematic evaluation of post-transcriptional gene regulatory signatures, and provides quantitative evidence for microRNA-directed target mRNA decay. Chemical nucleotide-analog-derivatization provides an accessible, cost-effective, and scalable method for the rapid and transcriptome-wide analysis of gene expression dynamics in living cells.
SPEAKER:
Stefan L. Ameres, PhD
Group Leader, Institute of Molecular Biotechnology (IMBA), Vienna, Austria
FOR MORE INFORMATION:
Learn more about SLAMseq Metabolic RNA Labeling Kit for RNA-Seq:
https://www.lexogen.com/slamseq-metabolic-rna-labeling/
Learn more about Lexogen products:
https://www.lexogen.com
Learn more about Advances in Genome Biology and Technology (AGBT) Meeting:
http://www.agbt.org/the-general-meeting/
FOLLOW US:
Facebook - https://www.facebook.com/lexogen
Twitter - https://twitter.com/lexogen
LinkedIn - https://www.linkedin.com/company/lexogen-gmbh
Instagram - https://www.instagram.com/lexogen/
ABSTRACT:
Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, processing and decay. We developed thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAMseq), an orthogonal chemistry-based epitranscriptome-sequencing technology that uncovers 4-thiouridine (s4U)-incorporation in RNA species at single-nucleotide resolution and absolute stoichiometry. In combination with established metabolic RNA labeling protocols and coupled to standard, high-throughput RNA sequencing methods, SLAMseq provides rapid access to RNA expression dynamics. We show that SLAMseq reports the functional RNA polymerase II-dependent transcriptional output, enables the systematic evaluation of post-transcriptional gene regulatory signatures, and provides quantitative evidence for microRNA-directed target mRNA decay. Chemical nucleotide-analog-derivatization provides an accessible, cost-effective, and scalable method for the rapid and transcriptome-wide analysis of gene expression dynamics in living cells.
SPEAKER:
Stefan L. Ameres, PhD
Group Leader, Institute of Molecular Biotechnology (IMBA), Vienna, Austria
FOR MORE INFORMATION:
Learn more about SLAMseq Metabolic RNA Labeling Kit for RNA-Seq:
https://www.lexogen.com/slamseq-metabolic-rna-labeling/
Learn more about Lexogen products:
https://www.lexogen.com
Learn more about Advances in Genome Biology and Technology (AGBT) Meeting:
http://www.agbt.org/the-general-meeting/
FOLLOW US:
Facebook - https://www.facebook.com/lexogen
Twitter - https://twitter.com/lexogen
LinkedIn - https://www.linkedin.com/company/lexogen-gmbh
Instagram - https://www.instagram.com/lexogen/
Most popular related searches