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Media supplemented with SimPL also contained additional magnesium sulfate sufficient to achieve a final media concentration of 5.7 mM Mg. 3T3-L1 cells were passaged using standard techniques in each condition 10 times before seeding a 96-well plate for label-free cell counting with a BioSpa 8 automated incubator and Cytation 5 multi-mode reader. Data represent counts of cells present within imaged well center. ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
Data represent cell counts relative to the initial seeding density average of each condition obtained from label-free cell counting with a BioSpa 8 automated incubator and Cytation 5 multi-mode ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
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Environics systems deliver precise gas concentrations for a wide range of clinical and research applications. Tailored to your exact research specifications, the exceptional accuracy makes them the preferred choice for laboratories and research settings requiring reliable and repeatable gas ...
ByEnvironics, Inc. based in Tolland, CONNECTICUT (USA)
Protein arrays are exposed to enzymes to identify targets for post-translational modifications. Many key protein functions require the protein to undergo a post-translational modification (PTM), examples ranging from phosphorylations and glycosylations to histone modifications and ubiquitinations. HuProt human proteome microarrays can be used for profiling the targets of enzymes producing PTMs and have been used in studies of phosphorylation, glycosylation, sumoylation, ...
ByCambridge Protein Arrays Ltd. (CPAP) based in Cambridge, UNITED KINGDOM
Comprehensive antibody reactivity profiling: validate specificity, identify cross-reactions. The issue of antibody cross-reactivity is one that has often been often overlooked or sidelined in the past, with typical antibody QC involving evidence of binding to the intended target, while neglecting potential cross-reactivity. In recent years, however, many examples have come to light where poor validation of antibodies has led to misleading or erroneous conclusions for assays in which they are used as reagents, due ...
ByCambridge Protein Arrays Ltd. (CPAP) based in Cambridge, UNITED KINGDOM
Growth performance of human adipose-derived mesenchymal stem cells in media containing Simplified Platelet Lysate (SimPL) and fetal bovine serum (FBS). Cells were grown in standard DMEM/F12 basal media containing penicillin, streptomycin, and GlutaMAX™ with either 10% (v/v) FBS or 2% SimPL (left). Similarly, cells were grown in identical conditions except for the use of reduced-serum basal media (Gibco™ Advanced DMEM/F12) with either 2% FBS or 2% SimPL (right). Medias supplemented with SimPL also ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
Laser Force Cytology™ (LFC), rapid viral infectivity assay data correlates tightly with traditional plaque and TCID50 assays. Radiance® provides new information around host response and the viral life cycle, enabling a better understanding of cellular infection through the measurement of novel phenotypic ...
ByLumaCyte, Inc. based in Charlottesville, VIRGINIA (USA)
Identify interacting proteins for your DNA/RNA molecule of interest. Profiling of nucleic acid binding on HuProt human proteome microarrays can reveal interactions with the largest collection of individual human proteins available on a single slide. Nucleic acid-protein interactions regulate many aspects of gene expression and thus cell physiology and pathology. DNA-protein interactions occur via a range of binding motifs present in transcription factors and nuclear receptors (e.g. DNA-binding domains, zinc ...
ByCambridge Protein Arrays Ltd. (CPAP) based in Cambridge, UNITED KINGDOM
Cancer-targeted molecular probes have the potential to revolutionize surgery and diagnostics. Offering unparalleled flexibility and outstanding performance, FIRE epitomizes the cutting edge of this technology. FIRE’s quenched, substrate-based probe (qSBP) technology guides surgeons by ensuring probe signal is only seen when cancer is detected, clearly delineating tumor tissue from healthy tissue and virtually eliminating spurious background fluorescence common in other probes. ...
ByAkrotome Imaging, Inc. based in Cleveland, OHIO (USA)
Discover autoantigens in autoimmune diseases, delineate new candidate biomarkers. Autoantibodies are present in the blood of all healthy individuals. However, when directed at high levels against protein targets in specific tissues, autoantibodies can be a major cause of autoimmune diseases. They are also important biomarkers for diagnosis of these conditions. ...
ByCambridge Protein Arrays Ltd. (CPAP) based in Cambridge, UNITED KINGDOM
Förster resonance energy transfer (FRET) is a phenomenon of non-radiative transfer of the optical excitation from an excited electronic level of one molecule, called the donor, to a resonant electronic level of another molecule, called the acceptor. In ...
ByEdinburgh Instruments - TECHCOMP Group based in , UNITED KINGDOM
Locus has built an automated, generalizable platform that is systematically unlocking the power of bacteriophage and CRISPR-Cas3 across multiple therapeutic ...
ByLocus Biosciences, Inc. based in Morrisville, NORTH CAROLINA (USA)
FluidFM® for Virology - Get a unique control over your single virion-single cell in-vitro experiment! Typically, viral infections are studied in bulk experiments where many cells in culture are exposed to the virus simultaneously, making it difficult to study single virus entry events or the spread of the viral infection between individual cells. With the gentle, micro-channeled, and force feedback-controlled FluidFM Probes, single virions can be deposited onto selected cells allowing unprecedented control to ...
ByCytosurge AG based in Glattbrugg, SWITZERLAND
A critical factor in ensuring complete resection of a solid cancer tumor is the ability to determine where diseased tissue ends and healthy tissue begins. Our research, as well as our clinical collaborators, confirm that there is a pressing unmet clinical need: the ability to rapidly and globally assess the status of marginal tissue adjacent to a tumor while the patient is in the ...
ByAkrotome Imaging, Inc. based in Cleveland, OHIO (USA)
We provide noise monitoring instruments designed for hospitals, helping improve patient recovery and protect staff ...
ByPulsar Instruments plc based in Hunmanby, UNITED KINGDOM