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Dna Target Articles & Analysis
33 articles found
Understanding sgRNAs sgRNAs are short RNA sequences that guide the Cas9 protein to the target DNA. Typically, they consist of approximately twenty nucleotides complementary to the target DNA sequence, paired with a scaffold region that recognizes and binds to Cas9. ...
This system allows researchers to target specific DNA sequences within a genome, facilitating the addition, removal, or alteration of genetic information. ...
Researchers design guide RNA (gRNA) that directs the Cas9 nuclease to a specific genomic location. Once the target gene is located, Cas9 induces a double-strand break, leading to disruptions in the gene’s function. ...
The ability to design dsRNA specific to target genes has opened up new avenues for gene silencing technology. ...
Conventional therapies often fall short in their ability to selectively target cancer cells without impacting healthy tissues. Alfa Cytology’s small molecule PARPi development services are specifically designed to overcome these limitations by delivering bespoke, high-quality PARP inhibitors tailored to the unique molecular characteristics of various cancers. ...
This process involves deleting, inserting, or modifying DNA in the bacterial genome, providing a powerful tool for studying gene function and genetic manipulation. The process uses nucleases that have been engineered to target specific DNA sequences, where they introduce double-strand breaks (DSBs) in the DNA molecule. ...
The backbone of clonality analysis is DNA and RNA sequencing, the most precise methods of determining the genetic makeup of cells. ...
What is CRISPRi? The dCas9 fusion protein targets DNA sequences through sgRNA, but the inactive dCas9 cannot catalyze DNA. Instead, a protein fused to dCas9 manipulates the transcription of the target gene. When dCas9 is fused to the transcriptional repression domain Kruppel-associated box (KRAB), transcription is repressed, a ...
SUMOylation plays a key role in gene expression regulation, DNA repair, RNA processing and nuclear transport in the cell nucleus. ...
There are unlimited uses for point mutation cell lines at pre-defined loci, including functional assays, drug screening, FACS screening of membrane proteins, gene expression studies, gene therapy and antibody immunization boosting research. The RNA-guided DNA endonuclease Cas9 associates with a synthetic single guide RNA (gRNA) and cleaves double-stranded DNA ...
Protein fusion is a genetic recombination technique in which the DNA of an inactive peptide or protein chain segment to be grafted is recombined with the DNA of a drug and expressed together by engineered cells; no specialized grafting operations are required. ...
This process involves deleting, inserting, or modifying DNA in the bacterial genome, providing a powerful tool for studying gene function and genetic manipulation. The process uses nucleases that have been engineered to target specific DNA sequences, where they introduce double-strand breaks (DSBs) in the DNA molecule. ...
Recombinases are bacterial enzymes that can manage DNA sequences, yielding comprehensive, exact, and persistent gene control. Incorporating recombinase technology into AAVs develops unique rAAV particles that can target specific DNA sequences. Substantial strides have been made in constructing rAAV particles to transport the recombinase ...
Introduction to Microbial Epigenetics Epigenetics has been defined as the study of stable alterations in gene expression potentials that arise during development and cell proliferation, or alterations in DNA function without alterations in DNA sequence. Modern epigenetic features refer to the alteration of DNA and/or associated proteins without ...
The backbone of clonality analysis is DNA and RNA sequencing, the most precise methods of determining the genetic makeup of cells. ...
Fluorescent Microspheres for Nucleic Acid Detection Microspheres encoded with up-converting luminescent materials of different colors can be used to bind different reporter labels, and when bound to single-stranded DNA, the fluorescence intensity will be different. A scientist used a similar method to wrap up-converting luminescent nanoparticles inside polystyrene microspheres, ...
Considering that targets play a key role in delivering drugs to cancer cells, it is important to develop new targets or ADCs to expand new indications and gain a larger market space. ...
Previous research has shown that the TnpB protein may act like a pair of molecular scissors, cutting DNA with the help of a special type of non-coding RNA called omega RNA. But how RNA-guided DNA cleavage works, and its evolutionary relationship to the Cas12 enzyme, was unclear, prompting research from Nureki's lab. ...
Targeted region sequencing combines both target enrichment and NGS technologies. ...
An ideal ADC drug remains stable in blood circulation, accurately reaches the therapeutic target, and eventually releases the cytotoxic payload in the vicinity of the target (such as cancer cells). ...