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Genomics Sample Articles & Analysis

11 articles found

Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications   

Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications  

Our kinetic analyses indicate that while traditional reaction buffers containing 10mM Mg²⁺ are sufficient for plasmid templates, complex genomic DNA samples benefit from supplementation with 0.5-1.0mM Mn²⁺, which enhances the enzyme's processivity on supercoiled substrates. ...

ByCreative Enzymes


Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications   

Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications  

Our kinetic analyses indicate that while traditional reaction buffers containing 10mM Mg²⁺ are sufficient for plasmid templates, complex genomic DNA samples benefit from supplementation with 0.5-1.0mM Mn²⁺, which enhances the enzyme's processivity on supercoiled substrates. ...

ByCreative Enzymes


Epitranscriptomic Modifications and How to Detect Them

Epitranscriptomic Modifications and How to Detect Them

The RNA sequencing results are compared to the genome sequencing results of a specific sample, which then reveals the location of inosine modifications. ...

ByCD Genomics


Exploring the Diversity of Microbial Eukaryotes: Insights and Study Techniques

Exploring the Diversity of Microbial Eukaryotes: Insights and Study Techniques

One example is metagenomic sequencing, which allows researchers to sample all the genes present in a complex sample of organisms. Another technique is targeted next-generation sequencing, which focuses on sequencing a specific region of the genome, making sample identification feasible. ...

ByCD Genomics


Bioinformatics Workflow for Whole Genome Sequencing

Bioinformatics Workflow for Whole Genome Sequencing

Whole genome sequencing (WGS) has the capacity to greatly enhance genomic knowledge and understand mysteries of life by utilizing the most advanced genetic sequencing technologies. WGS can be used for variant calling, genome annotation, phylogenetic analysis, reference genome construction, and more. ...

ByCD Genomics


Targeted Region Sequencing in Human Disease Studies and Clinical Care

Targeted Region Sequencing in Human Disease Studies and Clinical Care

With the ability to rapidly produce large volumes of sequencing data, next-generation sequencing (NGS) enables researchers to obtain whole genome or targeted regions of samples. Targeted region sequencing is preferred by researchers and clinical doctors who focus on human diseases, which is based on the fact that genomics and epigenomics have ...

ByCD Genomics


Applications of Microbial Whole Genome Sequencing

Applications of Microbial Whole Genome Sequencing

They are generally divided into fungi, actinomycetes, bacteria, spirulina, rickettsia, chlamydia, mycoplasma and viruses. Microbial whole genome sequencing is an important tool for mapping genomes of novel organisms, finishing genomes of known organisms, or comparing genomes across multiple samples. Sequencing ...

ByCD Genomics


Long Read Sequencing Helps HLA Typing

Long Read Sequencing Helps HLA Typing

Based on long-read sequencing technology, whole human genome sequencing can accurately develop the genetic variation of DNA sequences between samples and reference genome or between individuals, such as structural variation (SV) and copy number variation (CNV), by using the obtained 10~20kb long reads to compare with the reference ...

ByCD Genomics


CD Genomics Perspective: High-Throughput Sequencing Approach to Explore the Human Microbiome

CD Genomics Perspective: High-Throughput Sequencing Approach to Explore the Human Microbiome

Whole genome sequencing (WGS) has been introduced sporadically in oral microbiome research because the low number of samples from the oral cavity does not lend itself to large-scale analysis. ...

ByCD Genomics


Mapk4 May Be a New Target for the Treatment of Triple-Negative Breast Cancer

Mapk4 May Be a New Target for the Treatment of Triple-Negative Breast Cancer

Recently, an article titled " MAPK4 promotes triple negative breast cancer growth and reduces tumor sensitivity to PI3K blockade" was published in Nature Communications. By analyzing public genome databases, the researchers found that a large number of triple-negative breast cancer patients express high levels of MAPK4, and in animal models, elimination of MAPK4 reduced the ...

ByCreative BioMart


How Mosaic Offers Free Unlimited Genomic Samples

How Mosaic Offers Free Unlimited Genomic Samples

Mosaic allows users to add as much genomic data (CRAM, BAM, VCF) as they want to their Mosaic projects. This is true for paid accounts but surprisingly this is also true for free accounts (if you haven't already you can sign up and go crazy right now). As far as I'm aware, we are the only genomic cloud platform that offers this or perhaps more accurately can offer this. We are able to do this ...

ByFrameshift Labs, Inc.

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