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Protein Expression Articles & Analysis
114 articles found
Techniques such as immunohistochemistry and in situ hybridization can be performed on these sections to study protein expression and gene activity, respectively. This molecular insight is crucial for understanding the pathophysiological processes underlying kidney diseases and for the development of targeted therapies. ...
What is Protein Acetylation? At its core, Protein acetylation is the addition of an acetyl group (—COCH₃) from acetyl-coenzyme A (acetyl-CoA) to a protein. ...
The recombinant form of ELANE protein is typically expressed in eukaryotic systems to preserve post-translational modifications and enzymatic activity. ...
Structure and Formation VLPs are typically composed of viral proteins that self-assemble into spherical or filamentous structures resembling native viruses. ...
Through its impact on translation efficiency and mitochondrial function tRNA methylation modification also promotes tumor cell proliferation by controlling cell cycle-related protein expression. Cell cycle progression within cells is a critical biological function whose stoppage and progression rely entirely on cell cycle-related protein ...
Recombinant protein expression is a cornerstone of modern biopharmaceutical development, providing the means to produce proteins with therapeutic potential, enzymes, and antibodies. ...
Stress response mechanisms: Differential expression of heat shock proteins, particularly GroEL and DnaK chaperones, correlates with viability post-lyophilization—a critical consideration when selecting freeze-dried powder formulations for research. ...
Each chemokine binds to a corresponding G-protein-coupled receptor (GPCR), enabling specific cellular responses depending on the receptor-ligand pairing. Applications of Chemokines and Recombinant Chemokines Recombinant chemokines—genetically engineered proteins produced via expression systems—have gained prominence in both ...
On the other hand, IHC is focused on identifying specific proteins within a tissue sample using antibodies. By employing various tags or enzyme-linked systems, IHC enables the visualization of protein expression patterns. ...
Immunohistochemistry: Frozen sections enable the study of protein expression within lung tissues. Specific antibodies can be used to target and visualize proteins associated with inflammatory responses, fibrosis, and cellular proliferation. Molecular Studies: Techniques such as in situ hybridization can be performed on frozen sections to ...
They possess a high transfection efficiency, making them ideal for gene expression studies, protein production, and functional assays. The use of HEK293T as the background for the Monkey DLL3 stable cell line enhances the ability to manipulate the genetic material effectively, enabling the expression of DLL3 in a controlled manner. ...
The process involves printing small spots of biological samples, which are then used for various types of assays, including gene expression profiling, protein interaction studies, and glycan analysis. ...
This mimetic action allows researchers to enhance or restore the expression of target genes that are otherwise downregulated in diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. ...
The principle is to clone one or several antigen genes of a pathogen into host cells, causing them to express the corresponding antigen protein. These proteins are then extracted for use in the vaccine. ...
Protein-free medium Protein-free media do not contain any protein, only non-protein components. Using protein-free media improves cell growth and protein expression compared to serum-supplemented media, and facilitates downstream purification of any expressed ...
By combining surface marker profiles with indexes and transcriptomes, the authors now have a map of each cell type that can be used for later identification. They also measured the expression of an additional 352 surface markers that were not used in the original isolation protocol and that might better differentiate cells in the future. Indexed sorting data allowed each ...
One promising strategy is to deliver genes that express tumor suppressor proteins or anticancer agents into cancer cells to induce cell death or inhibit their proliferation. ...
Recombinant protein vaccines are vaccines prepared by expressing and purifying the antigen proteins of pathogens in engineered cells (such as bacteria, yeast, mammalian cells, and insect cells) using genetic engineering technology. Compared with other types of vaccines, recombinant protein vaccines are more costly but have ...
For instance, in certain cancers, abnormal expression of sialic acid proteins may promote the invasiveness and metastatic ability of tumor cells.Methods for Detecting Sialic Acid ProteinsDetection of sialic acid proteins typically involves the utilization of their sialic acid structure at the end of the sugar chain. ...
Differentially expressed proteins refer to proteins that show significant changes in their expression levels in different biological samples or under different treatment conditions. ...