- Home
- Articles
- latamerica
- real time qpcr
Refine by
Real Time Qpcr Articles & Analysis
6 articles found
Western BlotThis method can be used to detect and quantify the relative levels of specific collagen in tissue or cell lysates.Molecular Biology Methods1. Quantitative Real-Time Polymerase Chain Reaction (qPCR)By measuring the mRNA level of the collagen gene, the expression level of collagen can be indirectly inferred. ...
Cell lysates were prepared as described in the “Preparation of cell lysates and WES™ analysis” section for WES™ analysis. 2.4 Virus titer determination The viral titers of IAV and SARS-CoV-2 viruses were determined using a real-time one-step multiplex RT-qPCR method. Briefly, viral nucleic acid extraction was performed ...
Multiplex real-time PCR: A guide to application In multiplex real-time PCR (qPCR), scientists amplify two or more target genes in the same reaction, using the same reagent mix. ...
All of the feed homogenates were tested by a PRRSV quantitative real-time RT-PCR (RT-qPCR). Seven groups of feed homogenates from Treatment I (NC)-Id,Treatment 2 (PC)-Id,Treatment 3 (Product)-1 d.Treatment 2 (PC)-4d,Treatment 3 (Product)-4d,Treatment 2 (PC)-7d, and Treatment 3 (Product)-7d were selected for pig bioassay.Twenty-one (10-day-old) ...
We exposed adult fathead minnows (Pimephales promelas) for 48 hours to triclocarban (1.4 µg/L), DEET (0.6 µg/L) or to a PPCP mixture consisting of atenolol (1.5 µg/L), caffeine (0.25 µg/L), diphenhydramine (0.1 µg/L), gemfibrozil (1.5 µg/L), ibuprofen (0.4 µg/L), naproxen (1.6 µg/L), triclosan (2.3 µg/L), progesterone (0.2 µg/L), triclocarban (1.4 µg/L), and DEET (0.6 µg/L). Quantitative ...
DNA and RNA viruses were detected by the real time quantitative polymerase chain reaction (qPCR) and the reverse transcription-qPCR (RT-qPCR), respectively. ...