Antibiotic Selection Articles & Analysis
6 articles found
Development of the Monkey DLL3 Stable Cell Line The creation of a stable cell line involves several steps: Transfection: The HEK293T cells are transfected with a plasmid containing the DLL3 gene, along with appropriate selection markers. This initial step allows the cells to incorporate the DLL3 gene into their genome. Selection: After transfection, the cells are ...
The structure of a fosmid consists of several key components: an origin of replication, a selectable marker (usually an antibiotic resistance gene), and cloned DNA fragments. ...
Following this introduction, cells are selected based on successful integration of the reporter gene, often using antibiotic selection markers included in the vector. ...
Rapid pathogen identification aids in providing clinical diagnosis and selecting appropriate antibiotic treatment. It is also used in routine identification of bacteria in clinical laboratories to retrieve clinically relevant information to determine the role of microorganisms in disease, health, pathogenicity, epidemiology, etc. ...
In this study, a simple method based on wastewater-based epidemiology was adopted to estimate their usage in four megacities of China. Six antibiotics (norfloxacin, ofloxacin, sulfamethoxazole, trimethoprim, erythromycin and roxithromycin), which are the most frequently consumed antibiotics in China, were selected as the targets. Based on our ...
The release of antimicrobial substances into surface waters is of growing concern due to direct toxic effects on all trophic levels and the promotion of antibiotic resistance through sub-inhibitory concentration levels. This study showcases (1) the variation of antibiotics in sanitary sewage depending on different timescales and (2) a method to assess the ...