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Environmental Sampling Articles & Analysis
16 articles found
Our kinetic analyses indicate that while traditional reaction buffers containing 10mM Mg²⁺ are sufficient for plasmid templates, complex genomic DNA samples benefit from supplementation with 0.5-1.0mM Mn²⁺, which enhances the enzyme's processivity on supercoiled substrates. ...
Our kinetic analyses indicate that while traditional reaction buffers containing 10mM Mg²⁺ are sufficient for plasmid templates, complex genomic DNA samples benefit from supplementation with 0.5-1.0mM Mn²⁺, which enhances the enzyme's processivity on supercoiled substrates. ...
The process of 16S/18S/ITS amplicon sequencing entails DNA extraction from environmental samples, careful selection of universal primers to amplify the target region of 16S/18S/ITS, and the subsequent detection of sequence variations and abundance in the target region. This facilitates the study of differences in environmental microbial diversity ...
Viral metagenomics is the study of viruses in environmental and biological samples by utilizing next generation sequencing that generates very large data sets. ...
Metagenomics presents a powerful tool to study prokaryotes and viruses in the environment via the analysis of their DNA obtained directly from environmental samples. This technology considers the DNA of microbes in a population as a whole. ...
Metatranscriptomic sequencing provides direct access to culturable and non-culturable microbial transcriptome information by large-scale, high-throughput sequencing of transcripts from all microbial communities in specific environmental samples. Metatranscriptomic sequencing offers an opportunity to randomly sequence mRNAs as a unit for understanding the ...
Due to its low cost, shorter cycle and low quality requirement of the sample, 16S/18S/ITS amplicon sequencing is widely used in the study of microorganisms. 16S/18S/ITS amplicon sequencing reveals the species of microorganisms in environmental samples and their differences, relative abundance, population structure and evolutionary relationships. ...
Metagenomics presents a powerful tool to study prokaryotes and viruses in the environment via the analysis of their DNA obtained directly from environmental samples. This technology considers the DNA of microbes in a population as a whole. ...
Viral metagenomics is the study of viruses in environmental and biological samples by utilizing next generation sequencing that generates very large data sets. ...
This method has been widely used for the rapid diagnosis of bacteria in clinical, environmental, and industrial samples. Rapid pathogen identification aids in providing clinical diagnosis and selecting appropriate antibiotic treatment. ...
In the FacilityPro system, automation is supported by viable, nonviable and environmental controls, sampling automation through recipes, batch identifiers and data filters, and configurable reports and data management. Recipes allow sampling to be performed on an automatic-basis, driven by the SOP. The system is designed to manage viable, ...
Bio‐equivalents (e.g., 17β‐estradiol or dioxin equivalents) are commonly employed to quantify the in vitro effects of complex human or environmental samples. However, there is no generally accepted data analysis strategy for estimating and reporting bio‐equivalents. ...
Molecular methods are increasingly applied for virus detection in environmental samples without rendering data on viral infectivity. Infectivity data are important for assessing public health risks from exposure to human pathogenic viruses in the environment. ...
Significant difficulties remain for determining whether human noroviruses (hNoV) recovered from water, food, and environmental samples are infectious. Three-dimensional (3-D) tissue culture of human intestinal cells has shown promise in developing an infectivity assay, but reproducibility, even within a single laboratory, remains problematic. ...
In the study discussed in this article, environmental samples were obtained to identify the prevalence of MRSA on surfaces in high school athletic training and wrestling facilities mats in nine rural Ohio high schools. ...
Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes is a method that is widely used to detect and quantify microorganisms in environmental samples and medical specimens by fluorescence microscopy. Difficulties with FISH arise if the rRNA content of the probe target organisms is low, causing dim fluorescence signals that are not ...