Gel Electrophoresis Articles & Analysis
13 articles found
Research and DevelopmentIn the research and development stage of new products, qualitative detection helps to understand the protein's expression, folding, and functionality, guiding subsequent improvements and optimizations.Main Methods of Qualitative Detection1. Polyacrylamide Gel Electrophoresis (SDS-PAGE)SDS-PAGE is a basic and widely used method to separate ...
Meanwhile, recombinant collagen used for cosmetics or drug delivery systems may be designed with a lower molecular weight (e.g., 30 kDa to 90 kDa) for ease of skin absorption or to achieve specific biodistribution.Determination of Molecular WeightThe determination of the molecular weight of recombinant collagen typically uses the following techniques:1. Gel Permeation ...
Mass spectrometry analysis can be further improved in accuracy through different techniques, such as Tandem Mass Spectrometry (MS/MS) and high-resolution mass spectrometry.2. Gel ElectrophoresisGel electrophoresis is a method that separates proteins or peptide segments according to their molecular size. In collagen peptide research, polyacrylamide ...
SDS-PAGE (Sodium Dodecyl Sulfate-PolyAcrylamide Gel Electrophoresis)The collagen content can also be estimated by SDS-PAGE, especially in purified samples. ...
Agar is a galactan derived from red algae and contains α-1,3-linked L-galactose and β-1,4-linked d-galactose. It forms a gel-like substance when mixed with water, making it ideal for applications in molecular biology, including gel electrophoresis, DNA isolation, and protein separation. l Carrageenan The structure of carrageenan, the ...
Besides these commonly used methods above, other strategies such as difference gel electrophoresis (DIGE), Isotope-coded affinity tag (iCAT), and sequential windowed acquisition of all theoretical fragment ions (SWATH) can also be used to quantify proteins. ...
Although with the emergence of molecular methods such as Terminal Restriction Fragment Length Polymorphism (TRFLP), Automated rRNA Intergenic Spacer Analysis (ARISA), Random Amplified Polymorphic DNA (RAPD), Denaturing Gradient Gel Electrophoresis, Single Strand Conformation Polymorphism (SSCP), microautoradiography (MAR) and Fluorescent in situ Hybridization ...
In the present study, the genetic damage induced by gamma radiation and repair capacity in the target cells, i.e., cell lines originating from breast cancer, were analysed using a micronucleus (MN) test and comet assay (single-cell alkaline gel electrophoresis). Two breast tumour cell lines, MCF-7 and T-47D, were irradiated in a 60Co source (0.722 Gy/min) with ...
Phylogenetic characterization of this microbial community was done via 16S rRNA gene clone library and gradient gel electrophoresis analyses. Fluorescence in situ hybridization was used to quantify populations of 'Dehalococcoides' and Archaea and to examine the colocalization of these two groups within culture bioflocs. ...
Quantitative real-time PCR showed that EPS exerted a moderate bifidogenic effect, which was comparable to that of inulin for some polymers but which was lower than that found for glucose. PCR-denaturing gradient gel electrophoresis of 16S rRNA gene fragments using universal primers was employed to analyze microbial groups other than bifidobacteria. ...
By comparing genome sizes estimated from the microarrays and pulsed-field gel electrophoresis, the amount of unknown DNA in each strain was estimated. ...
Domestic drain microcosms were stabilized for 5 months and then exposed to polyhexamethylene biguanide (PHMB) at 0.1, 0.2, and 0.4g liter–1 over 6 months and characterized throughout by differential culture, together with eubacterial-specific PCR-denaturing gradient gel electrophoresis. Additionally, MICs and minimal bactericidal concentrations (MBCs) for ...
Studies of clonality performed by using enterobacterial repetitive intergenic consensus sequence PCR, Box element PCR, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE) collectively indicated that the V. cholerae O1 and O139 strains had a clonal origin, whereas the non-O1, non-O139 strains belonged to different clones. ...