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In Vitro Articles & Analysis
236 articles found
There is currently no standardized in vitro model for assessing the effects of dry cannabis ...
Vascular Calcification Studies In vitro models of vascular calcification—such as cultured vascular smooth muscle cells (VSMCs) treated with phosphate—use recombinant MGP to assess its ability to prevent calcium deposition. ...
Enzyme Activity Assays and Inhibitor Screening Recombinant ELANE is essential for in vitro assays measuring proteolytic activity against synthetic and physiological substrates. ...
Traditional investigations of podocytes have faced challenges due to their limited in vitro lifespan. Primary podocyte cultures tend to proliferate for only a brief period, making it difficult to conduct long-term studies on their behavior or response to therapeutic agents. ...
One of the most widely recognized in vitro models for evaluating the intestinal absorption of compounds is the Caco-2 permeability assay. ...
Some studies have examined drug delivery systems using platelet endocytosis to absorb fluorescent beads and viral particles which get stored in platelet α-granules for subsequent release during platelet activation. During in vitro incubation researchers can administer interferon-γ-inducible protein 10 (IP10) inside platelets. ...
This includes selecting appropriate radioactive isotopes and designing linkers that will ensure stable attachment to the targeting moiety. 3. In Vitro and In Vivo Studies: Prior to human trials, extensive laboratory studies are conducted to evaluate the efficacy, stability, and safety of the conjugate. ...
The ability to manipulate these cells in vitro enables a deeper understanding of their role in cerebellar health and disease. ...
Traditionally, drug discovery relied on simple in vitro systems or animal models that often failed to replicate the complexity of human diseases. ...
Animal models offer researchers valuable insights that cannot be obtained through in vitro studies alone, bridging the gap between laboratory research and human application. ...
These approaches often include in vitro and in vivo testing, as well as computational modeling. In vitro testing involves studying immune responses in controlled laboratory environments using cell cultures. ...
Moreover, metabolic flux assays, which implement specific experimental designs and tools to measure fluxes, complement MFA by standardizing and refining this dynamic analysis in both in vitro and in vivo systems. How Do Metabolomics Services Complement MFA? ...
The ability to design dsRNA specific to target genes has opened up new avenues for gene silencing technology. In Vitro Transcription One of the most common methods for producing dsRNA is in vitro transcription. ...
Such models allow for controlled studies in vitro, facilitating the examination of cellular mechanisms, lipid metabolism, and the effects of specific compounds on liver cells. ...
Once isolated, hMSCs can be cultured in vitro under specific conditions that support their proliferation while maintaining their stemness. ...
Types of ADME Testing Services To ensure comprehensive evaluation, various testing methodologies are employed, including in vitro and in vivo studies, alongside computational modeling techniques. In vitro tests often involve using cell cultures or biological fluids to assess how drugs are absorbed and metabolized. ...
It has been established in vivo and in vitro experiments and reviews that dual-target peptide-modified RBCNPs exhibit better targeted uptake of gliomas than nanoparticle-based drug delivery systems, with beneficial and safe therapeutic outcomes, which indicates the high potential of bionic nanomaterials in the treatment of tumors. ...
Preclinical evaluation of cardiovascular safety involves a multifaceted approach, encompassing a plethora of in vitro and in vivo endpoints. These assessments continuously evolve, aiming to mitigate the incidence of cardiovascular toxicity that may emerge during clinical trials. ...
Recognizing the need for a more accurate and efficient evaluation system, the Comprehensive in vitro Proarrhythmia Assay (CiPA) initiative emerged. At the heart of this initiative are CiPA Translational Assays, designed to improve the prediction of drug-induced cardiac arrhythmias. ...
They can reveal the time-dependent relationship between drug concentration and its effects, offering a comprehensive overview that in vitro or in silico studies alone cannot provide. Challenges and Future Perspectives Conducting in vivo PK/PD studies poses several challenges, including ethical considerations, variability in biological responses, and the high costs associated with ...