Molecular Spectroscopy Articles & Analysis
1 articles found
When the PCR cycle reaches the extension phase, the Taq polymerase primer nuclease activity on the 5’ end ruptures the FAM from the BHQ-1 in the probe and its fluorescence is then measured as a function of each cycle, increasing in intensity for positive diagnoses.While RT-PCR test kits and molecular probes have become more available in recent years, these molecules took creative ...