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Protein Expression Articles & Analysis
82 articles found
Rare diseases collectively affect hundreds of millions of people worldwide, yet many individual conditions remain difficult to diagnose and treat due to their low prevalence and complex biological mechanisms. In recent decades, therapeutic enzymes have emerged as an important class of biologic drugs that address the underlying causes of several rare genetic disorders. By replacing or ...
Introduction The BALB/C mouse is a widely used inbred strain in biomedical research, particularly in the fields of immunology, cancer research, and genetics. Among the various tissues studied, the kidney holds significant importance due to its complex structure and multifaceted role in physiological functions. A formalin-fixed paraffin-embedded (FFPE) block of the BALB/C mouse kidney serves as an ...
In the intricate world of cellular biology, proteins are the undisputed workhorses. They are assembled based on a genetic blueprint, but their story doesn't end there. After synthesis, proteins undergo a vast array of chemical modifications known as post-translational modifications (PTMs). These changes act as molecular switches, fine-tuning a protein's function, localization, and stability. ...
Virus-like particles (VLPs) have garnered significant attention in recent years for their potential applications in vaccine development, drug delivery, and as tools in various biotechnological fields. These nanoparticles mimic the structure of viruses but lack the viral genetic material that makes viruses infectious. This unique characteristic offers a versatile platform for various innovative ...
Post-transcriptional modifications of RNA molecules include methylation, acetylation deamination, isomerization, glycosylation, thiolation and pseudouracil modification among other types. Methylation represents the predominant post-transcriptional modification found within transfer RNA (tRNA) molecules. tRNA methylation has maintained its evolutionary conservation while also exhibiting dynamic ...
Recombinant protein expression is a cornerstone of modern biopharmaceutical development, providing the means to produce proteins with therapeutic potential, enzymes, and antibodies. Among various expression systems, Chinese Hamster Ovary (CHO) cells have emerged as the preferred choice for many researchers and pharmaceutical companies. This article delves into the significance of recombinant ...
Fluorescence In Situ Hybridization (FISH) and Immunohistochemistry (IHC) are two powerful techniques widely used in biological and medical research. While each has its strengths, the combination of these methods—ImmunoFISH—offers a unique and comprehensive approach to investigation at the cellular and tissue levels. Understanding the Techniques FISH is primarily utilized for detecting ...
Research in the field of pulmonary biology often requires detailed analysis of lung tissues. One of the optimal methods for studying lung structures and functions in mice is the use of C57 mouse models, specifically through the preparation of frozen sections. This technique is vital for a variety of applications, including histological studies, immunohistochemistry, and molecular analysis. Why ...
In the rapidly evolving field of cellular biology and genetic engineering, the development of efficient and reliable cell lines is vital for research and therapeutic applications. One such advancement is the establishment of the Monkey DLL3 stable cell line using HEK293T cells. This article provides an overview of the significance of this cell line, its applications, and the methodology behind ...
Recombinant protein vaccines are vaccines produced using genetic engineering technology. The principle is to clone one or several antigen genes of a pathogen into host cells, causing them to express the corresponding antigen protein. These proteins are then extracted for use in the vaccine. These vaccines can effectively stimulate the immune system to respond to specific pathogens, thereby ...
Human brain has approximately 171 billion cells, of which slightly more than half (approximately 86 billion) are nerve cells. These 86 billion nerve cells are a diverse cell population with hundreds of specialized types and functions, but all originate from three neural cell lineages--neurons, oligodendrocytes and astrocytes. All three cell lineages originate from a pool of neural stem and ...
Cell culture is one of the major technologies in the life sciences. It is a general term used to remove cells, tissues or organs from animals or plants and place them in an artificial environment conducive to their survival and/or proliferation. The basic environmental requirements for optimal cell growth are: controlled temperature, substrate for cell attachment, appropriate growth medium and ...
Gene delivery is a therapeutic strategy that’s revolutionizing biotechnology and pharmaceuticals. It involves the delivery of new genetic molecules into target cells to treat diseases. This technology has the implicit to revise the treatment of inherited genetic disorders, cancer and other intractable driseases. Scientists are using a variety of gene delivery strategies to insert new ...
Differentially expressed proteins refer to proteins that show significant changes in their expression levels in different biological samples or under different treatment conditions. Observing such differential expression is crucial for understanding cellular physiology and disease mechanisms. For example, some proteins may be upregulated or downregulated in health and disease states, or between ...
Plasma proteomics is an emerging field of science dedicated to the analysis and understanding of the protein composition in the plasma and its functions. Plasma, as one of the most abundant biological fluids in the human body, contains tens of thousands of proteins, reflecting the health status, disease process, and treatment response of the body. Therefore, plasma proteomics holds great ...
The immune system plays a crucial role in protecting the body from harmful pathogens and foreign invaders. Immune receptors, which are protein molecules expressed on the membrane of immune cells, play a key role in regulating immune responses. These receptors can interact with specific ligands to trigger a cascade of events that lead to the activation or suppression of immune responses. Immune ...
In-depth research on protein interaction plays a crucial role in life science research. Proteins are the main bearers of life activities, and their interactions form a complex molecular network within biological cells. Protein-Protein Interaction Sequencing (PPI-Seq) is an emerging technology. Based on high-throughput sequencing, it allows researchers to explore protein interactions at the whole ...
Brain tumors can be broadly categorized into primary and secondary (or metastatic) tumors. Primary tumors originate in the brain1, while secondary tumors spread from other body parts. Treatment options traditionally include surgery, radiation therapy, and chemotherapy. However, these approaches can be invasive and have severe side effects. The battle against brain tumors is being ...
Collagen, as one of the most abundant proteins in the human body, plays a key role in maintaining the integrity and function of tissue structures. With the development of bioengineering technology, recombinant human collagen (rhCollagen) produced by recombinant DNA technology has been widely applied in various fields such as medicine, cosmetics, and tissue engineering. Therefore, qualitative ...
In the fields of cell biology and genetic engineering, gene knockout is a commonly used research method that can help us understand the function and role of a specific gene, and the efficiency of this process is critical. Gene knockout is a procedure that inactivates the function of a specific gene, making it unable to express normally, through genetic engineering techniques. This operation can ...