Reaction Optimization Articles & Analysis
6 articles found
In the fast-evolving field of antibody–drug conjugates (ADCs), linker chemistry plays a decisive role in defining therapeutic performance. The ability to control the drug-to-antibody ratio (DAR), conjugation site, and overall molecular stability directly impacts both efficacy and safety. Among emerging linker materials, azide-functionalized PEG derivatives have become essential tools for ...
This method involves the stepwise construction of lipid molecules through a series of chemical reactions, allowing for the incorporation of various functional groups. ...
Most of these lipids are synthesized by Michael addition or epoxy ring-opening reaction, and they cannot be biodegraded and may result in cytotoxicity if they accumulate to a certain amount in the body. In addition, these lipids often have the disadvantage of low chemical yield after being synthesized by the multi-step reaction. To optimize LNP ...
Factors Affecting Restriction Endonuclease Activity (1) DNA purity: Impurities in DNA such as protein, phenol, chloroform, ethanol, SDS, EDTA, etc. will affect the activity of the enzyme, which is generally improved by purifying DNA, increasing the amount of enzyme, prolonging the incubation time and expanding the reaction system. (2) Degree of DNA methylation: ...
Pharmaceutical excipients are natural or synthetic substances formulated together with the active ingredients of the drug, whose purpose is to increase the formulations containing effective active ingredients or endow the active ingredients in the final dosage form with therapeutic effects, such as promoting drug absorption or solubility. Pharmaceutical excipients can also be used in the ...
In this method stannous chloride is used as reducing agent and potassium sodium tartrate as transchelation agent. Optimal parameters of the labelling reaction and also, the stability of the radiolabelled nanospheres were established. Under optimal reaction condition, after 45 min. at 75°C, the labelling yields were more than 95%; ...