Sample Separation Articles & Analysis
9 articles found
This dual-color or multiplex approach enriches the data obtained, offering detailed insights into the relationships between different molecular targets within the same sample. One key feature of our ISH services is the capability for multiplex ISH, which allows for the localization of two or more probes within a single sample. ...
This article mainly introduces how to detect the ubiquitination level of a protein.ImmunoblottingImmunoblotting is a common method for detecting the level of protein ubiquitination, mainly through the detection of ubiquitinated proteins in protein samples using specific antibodies. Firstly, the protein sample is separated by SDS-PAGE, then the ...
In Western Blot and Far-Western Blot, proteins are separated from the samples by SDS-gel and native PAGE gel, and then the proteins are transferred from the gel to the membrane. ...
A typical GWA study has four parts: 1) a large number of people with the disease or trait of interest and a suitable control group; 2) DNA isolation, genotyping, and data analysis to ensure high genotyping quality; 3) statistical tests for associations between SNPs that pass quality thresholds and disease/trait; and 4) replication of found associations in a separate ...
Ion chromatography and sample filtration go hand-in-hand since this pretreatment step is advised for the majority of sample matrices. It is essential to avoid injecting particles, algae, or bacteria into the IC system, as these can jeopardize both the instrument and the separation column. Samples are often prepared manually, for ...
ByB&W Tek
However, there is an increased risk of cross contamination since all samples are contained within a shared liquid. If a sample bag or vial were to split or leak, then the contamination could easily spread to the other samples. ...
Introduction of HPLC-MS/MS-based Proteomics Technology The development of high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) based proteomics technology mainly depends on the liquid chromatography-mass spectrometry equipment, sample separation and preparation technology, identification, as well as bioinformatics. ...
We generated a prediction model using 153 samples, with validation using an additional 58 clinical outcome-blinded samples. Gene expression profiles were generated using formalin-fixed, paraffin-embedded tissue samples and microarrays. Data analysis was performed using a supervised clustering method. The prediction model generated from mixed ...
If there is too little bile acid or lecithin in the liver, or too much cholesterol, cholesterol precipitation is likely to occur and gallstones are formed. Detection of Bile Acids The separation of bile acids is particularly complicated by the presence of structural analogs (as isomers) and the polarity differences between unconjugated and conjugated bile acids. ...