Thermal Cycler Articles & Analysis
7 articles found
RNA isolated and purified from samples of the upper and lower respiratory tract is first subjected to reverse transcription to cDNA, then amplified in real-time thermal cycler PCR. Reporter dye at 5 'and quenching dye at 3' are the probes. ...
As the world opens up over the coming months and years, there will be countless plane journeys to see loved ones, meet new family members and travel for work and leisure. Many of these travellers will first need to provide evidence of a negative PCR test to show they pose a minimal COVID-19 transmission risk. Time is proving of the essence for testing. With reports of multi-hour queues at ...
Mic qpcr cycler and Myra liquid handler have been put through their paces in COVID-19-testing field trials in regional Western Australia and found to be noticeably robust, rapidly deployable and flexible. ...
In 2020 Analytik Jena’s real-time PCR thermal cyclers of the qTOWER³ series even supported one of the first SARS-CoV-2 wastewater screenings in Japan. ...
Overview of the SARS-CoV-2 (Coronavirus) Detection Process In December 2019 the novel SARS-CoV-2 (formerly named 2019-nCoV) was identified in Wuhan, the capital of China’s province Hubei. Based on its rapid spreading with more than 105,000,000 confirmed cases by beginning of February 2021 the World Health Organization declared the outbreak a public health emergency of international ...
Multiplex real-time PCR: A guide to application In multiplex real-time PCR (qPCR), scientists amplify two or more target genes in the same reaction, using the same reagent mix. Multiplexing allows scientists to overcome limitations for myriad applications. First and foremost, multiplexing enables scientists to conserve valuable samples used in a quantitative assay, which is especially ...
Real-time PCR was performed in a Rotor-Gene Q (Qiagen, Hilden, Germany) thermal cycler using the QuantiTect SYBR Green PCR kit (Qiagen) with the primers (0.01 mM) listed in Table S1. ...