Secondary Antibody Articles & Analysis
4 articles found
Western blot can accurately detect the presence and expression of specific proteins in samples, and it can also be used to detect protein post-translational modifications, such as phosphorylation, ubiquitination, etc. The overall workflow of Far-Western Blot technology is similar to Western Blot, but the detection of target proteins in Western Blot requires antibodies, while antibodies are not ...
From protein immunoblotting to immunofluorescence, and then to FRET, antibodies are often an important determinant of successful and repeatable experiments. But we've never had a set "plan" to find the most suitable antibody. In this issue, the editor shares a 10-step "plan" for antibody selection.Determine the Name of the Target ProteinFirst, you need to be clear about what protein you need to ...
Antibodies can often be used in multiple assays, but they do not perform equally well in all assays. This is particularly true for immunohistochemistry (IHC). IHC has now become an indispensable assay and is consistently performed in anatomic pathology to give specific diagnosis and subclassification of neoplasms. IHC is a complex assay, where the end result is influenced by a variety of ...
Collagen is a primary structural protein in various tissues, especially in the skin, bones, tendons, and ligaments. The detection of collagen content can assist in evaluating the progression or treatment effectiveness of certain diseases, as well as assessing the quality of cosmetics and food. Methods for Detecting Collagen Content 1. Hydroxyproline Assay (1) Collagen is one of the few proteins ...