Creative Bioarray products

Most tissues are composed of multiple cell types organized in the three-dimensional (3D) arrangement required for intercellular communication and function. In vitro fabrication of living tissue involves recapitulating this complex cellular structure, which is difficult to do in a controlled manner. To fully realize artificial, cell-laden biological models in tissue engineering, such as 3D organoids and organ-on-a-chip systems, cells need to be patterned so that they can accurately mimic natural microenvironments in vitro. Despite the growing interest in this field, cell patterning at multiple scales (~10 μm to 10 mm) remains a major challenge in bioengineering.

Recommended Medium-RPMI Advanced medium supplemented with 2% fetal bovine serum.

Internucleosomal DNA fragmentation is a hallmark of Apoptosis in mammalian cells. Creative Bioarray`s SuperDirect® DNA fragmentation assay kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTPAt the free 3`-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be observed by fluorescence microscopy orAnalyzed by flow cytometry. Storage-20°C.

Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. SuperQuick mitochondrial apoptosis detection fluorometric kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. Storage-20°C.

The SuperQuick® annexin V-Biotin apoptosis detection kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, aprotein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using Any streptavidine- or Avidin-dye reagents.

SuperQuick® annexin V apoptosis detection kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate ofA nnexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy.

SuperQuick annexin V apoptosis detection kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining withA fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. Detection can beAnalyzed by flow cytometry or by fluorescence microscopy.

The SuperQuick annexin V-EGFP apoptosis detection kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with an enhanced green fluorescent protein (EGFP) fusion ofAnnexin V, a protein that has a strong natural affinity for PS. EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-EGFP and PI staining. Storage : 4°C.

SuperQuick annexin V apoptosis detection kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate ofAnnexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both annexin V-FITC and PI staining. Storage : 4°C