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Cells Using 3D Bioprinting Traps

Most tissues are composed of multiple cell types organized in the three-dimensional (3D) arrangement required for intercellular communication and function. In vitro fabrication of living tissue involves recapitulating this complex cellular structure, which is difficult to do in a controlled manner. To fully realize artificial, cell-laden biological models in tissue engineering, such as 3D organoids and organ-on-a-chip systems, cells need to be patterned so that they can accurately mimic natural microenvironments in vitro. Despite the growing interest in this field, cell patterning at multiple scales (~10 μm to 10 mm) remains a major challenge in bioengineering.